Analysis of curcumin and piperine in biological samples by reversed-phase liquid chromatography with multi-wavelength detection

Widely accessible food phytochemicals such as curcumin have been reported to anti-inflammatory and anticarcinogenic properties. However, has poor absorption in the gut, piperine of interest a dietary compound that can enhance bioavailability. The aim this study was develop optimize technique using reversed-phase chromatography with multi-wavelength detection for simultaneous measurement various biological matrices. Emodin used an internal standard. Protein precipitation liquid-liquid extraction based on acetonitrile provided good recovery these analytes. A 150 mm × 4.6 I.D. Luna C18 column under isocratic conditions separate curcumin, piperine, emodin baseline resolution, separation from other sample components, little 4 min. limits were 3 7 ng/mL, respectively. This method quantitate compounds samples human intestinal epithelial cell lysates mouse plasma or GI tissues research aimed at examining bioavailability presence piperine.